SignaIDT Biotechnologies (SZ), Inc. has successfully developed the first freeze-dried real-time fluorescent RT-PCR detection reagent for new coronavirus in China. The reagent is a multiple real-time fluorescent RT-PCR detection reagent that uses the N gene sequence of the new coronavirus for primer probe design, and the human RNAse P gene as an internal standard. It does not require cold chain transportation and storage, and the transportation and management costs are greatly reduced. Eliminate losses caused by cold chain failure, pipetting errors, repeated freezing and thawing, aerosol pollution, etc., single-serve packaging, easy to use, no need to wait, especially suitable for primary medical institutions with poor epidemic prevention and control and cold chain conditions .

According to Dr. Li Shaoji, R&D Director of Segno Biology, "The most important feature of this product is that the design of primers and probes avoids the main defects of the primers and probes published by WHO, including unreasonable Tm values. , unreasonable relative positions of probes and primers, stable secondary structures (especially stable probe hairpins), etc., ensure the maximum utilization efficiency of reagent components in the amplification process, and contribute to the optimization and improvement of the reaction system The assay sensitivity of the reagent removes the inherent barrier."

Dr. Lin Jingzhong, CEO of Segno Bio, said: "The outbreak of the new coronavirus is a major test for China's medical and health system. Nucleic acid detection (real-time fluorescent RT-PCR) and high-throughput sequencing are important in the prevention and control of this epidemic. The contribution is indispensable, but we also see that these advanced detection technologies can only be applied in some large-scale medical institutions at present, and grass-roots medical institutions and even intermediate-scale medical institutions have comparative advantages in terms of technology, talents, detection capacity, and management. There is a huge shortage. After the epidemic, the country will inevitably increase its efforts to develop the medical industry, and the sinking of advanced technology is an inevitable trend. Our products do not require cold chains, have low transportation and management costs, are easy to use, and have great potential in the application of grassroots medical institutions. It has obvious advantages and broad application prospects.”

Segno Bio is a high-tech biological enterprise specializing in the development and production of molecular detection reagents. It is the earliest in vitro diagnostic reagent enterprise in my country dedicated to the development of freeze-dried molecular detection reagents that do not require a cold chain. All fields have independent intellectual property rights, and the technology has reached the domestic leading and international advanced level. In addition to the new coronavirus, the company has formed dozens of products in multiple detection series such as molecular diagnosis, food pathogens, animal epidemics, and disease prevention and control. more favorable position.

Lin Jingzhong said: "There are many respiratory infection pathogenic bacteria that cause symptoms similar to the new type of coronavirus pneumonia, including influenza virus, SARS coronavirus, MERS coronavirus, respiratory syncytial virus, human infection with avian influenza virus H7N9, etc. The new type of coronavirus The successful development of freeze-dried real-time fluorescent RT-PCR detection reagents, combined with the successful development of influenza virus and other detection reagents, has laid a solid foundation for us to further develop products that simultaneously detect multiple respiratory tract infections."

Graphic: Novel coronavirus freeze-dried nucleic acid detection reagent (real-time fluorescent RT-PCR method) is a reagent that does not require cold chain transportation and storage. It is packaged for one person (left picture), easy to use, no waiting, no loss, and low cost Low. Using the N gene of the new coronavirus to design specific primers and probes, and using the human RNAse P gene as an internal standard, the amplification efficiency is high, the linear range is wide, and the detection sensitivity is high (right figure).